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Table 2 Dissociation constants (K D ) of rEhTBP and rEhTRF1 for different TATA variants and GAAC-box

From: The Entamoeba histolytica TBP and TRF1 transcription factors are GAAC-box binding proteins, which display differential gene expression under different stress stimuli and during the interaction with mammalian cells

DNA sequence rEhTBP rEhTRF1
TATTTAAA (1) (2.07 ± 0.49) × 10-11 M (5.29 ± 0.98) × 10-11 M
TAgTgAAA (2) (3.98 ± 0.16) × 10-11 M (1.30 ± 0.51) × 10-11 M
TATTggAA (3) (3.57 ± 0.13) × 10-11 M (1.35 ± 0.88) × 10-11 M
TATTaAAA (4) (4.85 ± 3.06) × 10-12 M (3.98 ± 1.96) × 10-12 M
TATgTAAA (5) (1.69 ± 1.37) × 10-12 M (6.74 ± 2.25) × 10-12 M
gAgTTAAA (6) (4.73 ± 2.91) × 10-12 M (6.85 ± 4.77) × 10-12 M
TAcTcAAA (7) (2.76 ± 1.48) × 10-12 M (2.98 ± 1.37) × 10-11 M
cAcTcAAA (8) (3.80 ± 1.46) × 10-11 M (3.08 ± 1.15) × 10-11 M
cAcTTAAA (9) NB (9.72 ± 4.88) × 10-12 M
TATTTttt (10) (3.40 ± 1.64) × 10-12 M (2.54 ± 1.37) × 10-11 M
GAAC-box (1.90 ± 0.16) × 10-11 M (7.94 ± 1.44) × 10-11 M
  1. Abbreviation: NB No binding activity for this DNA sequence