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Table 2 Dissociation constants (K D ) of rEhTBP and rEhTRF1 for different TATA variants and GAAC-box

From: The Entamoeba histolytica TBP and TRF1 transcription factors are GAAC-box binding proteins, which display differential gene expression under different stress stimuli and during the interaction with mammalian cells

DNA sequence

rEhTBP

rEhTRF1

TATTTAAA (1)

(2.07 ± 0.49) × 10-11 M

(5.29 ± 0.98) × 10-11 M

TAgTgAAA (2)

(3.98 ± 0.16) × 10-11 M

(1.30 ± 0.51) × 10-11 M

TATTggAA (3)

(3.57 ± 0.13) × 10-11 M

(1.35 ± 0.88) × 10-11 M

TATTaAAA (4)

(4.85 ± 3.06) × 10-12 M

(3.98 ± 1.96) × 10-12 M

TATgTAAA (5)

(1.69 ± 1.37) × 10-12 M

(6.74 ± 2.25) × 10-12 M

gAgTTAAA (6)

(4.73 ± 2.91) × 10-12 M

(6.85 ± 4.77) × 10-12 M

TAcTcAAA (7)

(2.76 ± 1.48) × 10-12 M

(2.98 ± 1.37) × 10-11 M

cAcTcAAA (8)

(3.80 ± 1.46) × 10-11 M

(3.08 ± 1.15) × 10-11 M

cAcTTAAA (9)

NB

(9.72 ± 4.88) × 10-12 M

TATTTttt (10)

(3.40 ± 1.64) × 10-12 M

(2.54 ± 1.37) × 10-11 M

GAAC-box

(1.90 ± 0.16) × 10-11 M

(7.94 ± 1.44) × 10-11 M

  1. Abbreviation: NB No binding activity for this DNA sequence
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