Fig. 4

Analytical sensitivity and specificity of Tc189F/Tc189R primers to trypanosomatids and other microorganisms. PCR assays using primers Tc189F/Tc189R were performed with two ng DNA of trypanosomatids, yeast, fungi and bacteria. a Specificity assay (Non-detectable PCR). Upper lanes - Lane 1: 100 bp DNA ladder; Lane 2: sterile water (negative control); Lane 3: T. rangeli; Lane 4: T. cervi; Lane 5: T. lewisi; Lane 6: T. mega; Lane 7: L. amazonensis; Lane 8: L. braziliensis; Lane 9: 100 bp DNA ladder; Lane 10: no template. Bottom lanes - Lane 1: L. guyanensis; Lane 2: L. lainsoni; Lane 3: L. naiffi; Lane 4: L. shawi; Sensitivity assay (detectable PCR). Lane 5: 100 bp DNA ladder; Lane 6: T. cruzi Dm28c clone (TcI); Lane 7: T. cruzi Y strain (TcII); Lane 8: T. cruzi INPA 222 strain (TcIII); Lane 9: T. cruzi CL Brener clone (TcVI); Lane 10: 100 bp DNA ladder. b Specificity assay (continuation). Lane 1: 100 bp DNA ladder; Lane 2: A. alternate; Lane 3: B. cinerea; Lane 4: O. polymorpha; Lane 5: S. cerevisae; Lane 6: B. cereus; Lane 7: C. sakasaki; Lane 8: E. coli; Lane 9: Salmonella sp.; Lane 10: S. aureus; Lane 11: T. cruzi CL Brener clone (TcVI)