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Table 5 Dogs positive by the different PCRs performed and the corresponding IFAT results for different antigens studied

From: Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?

Dog

ID

qPCR

L. infantum

(parasite/ml);

ELISA dilution (EU)

IFAT

qPCR

Ehrlichia/ Anaplasma

Conventional PCR

Ehrlichia/ Anaplasma

PCR

Hepatozoon/ Babesia

Clinical signs and laboratory abnormalities

Rc

Ec

Ap

Bh

HCV-7

Negative (0);

Positive (195)

512

1024

1024

256

Positive

Negative

Negative

Moderate lymphadenomegaly,

skin lesions. Thrombocytopenia, hypercholesterolemia, hyperproteinemia, hyperbetaglobulinemia

HCV-9

Positive (51);

Positive (39892)

< 64

< 64

< 64

256

Positive

Negative

Negative

Fever, weight loss, moderate lymphadenomegaly, ocular lesions. Pancytopenia, hypoalbuminemia, hypergammaglobulinemia

HCV-11

Positive (4);

Positive (12568)

> 512

< 64

64

128

Positive

Negative

Negative

Skin lesions. Hypoalbuminemia, hypergammaglobulinemia, proteinuria

HCV-28

Positive (807);

Positive (30827)

512

1024

256

< 64

Positive

Negative

Negative

Weight loss, moderate lymphadenomegaly,

skin and ocular lesions. Regenerative anemia, hypoalbuminemia, hypergammaglobulinemia, proteinuria

HCV-31

Positive (8);

Positive (37360)

64

64

128

64

Positive

Negative

Negative

Fever, mild lymphadenomegaly, epistaxis. Anemia, lymphocytosis, thrombocytopenia, hypoalbuminemia, hypergammaglobulinemia

MO-1

Positive (33);

Positive (47)

512

128

64

< 64

Positive

Positivea

Negative

Skin lesions. Hypercholesterolemia

MO-4

Negative (0);

Positive (188)

256

64

1024

< 64

Negative

Positivea

Skin lesions. Mild hyperbetaglobulinemia

MED-5

Negative (0);

Positive (7170)

64

< 64

< 64

256

Positive

Positivea

Negative

Weight loss, moderate lymphadenomegaly,

skin and ocular lesions, splenomegaly. Anemia, hypoalbuminemia

MED-7

Negative (0);

Positive (82)

< 64

< 64

1024

< 64

Positive

Negative

Negative

Moderate lymphadenomegaly,

skin lesions. Hypoalbuminemia

  1. Abbreviations: Ap Anaplasma phagocytophilum, Bh Bartonella henselae, Ec Ehrlichia canis, IFAT immunofluorescence antibody test, PCR polymerase chain reaction, Rc Rickettsia conorii, qPCR real-time PCR
  2. aThree pathogens were sequenced with the following results: two A. platys and one H. canis