Table 1 Target pathogens, molecular method, target genes and primers used in this study
Target | Method | Gene | Primer | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
B. burgdorferi (s.l.) | Traditional PCR | Flagellin | FLA1; FLA2 | 482 | [51] |
“Candidatus N. mikurensis” | Traditional PCR | groEL | NM-350s (5'-GTG TAA TGA CAA AGT TGG TGA TGG-3'); NM-1152as | 802 | This study; [52] |
A. phagocytophilum | SYBR green rPCR | msp2 | msp2-3f ; msp2-3r | 334 | [53] |
Rickettsia spp. | SYBR green rPCR | OmpB | rompB OFm; rompB ORm | 489 | [54] |
Babesia spp. | SYBR green rPCR | 18S rRNA | BJ1; BN2 | 411–452 | [55] |
DNA extraction control | Traditional PCR | 18S rRNA | 18SU; 18SD | 488 | [37] |
TBE virus | TaqMan rRT-PCR | 3' non-coding region | F-TBE 1; R-TBE 1 TBE-Probe-WT | 67 | [38] |
RNA extraction control | TaqMan rRT-PCR | 16S rRNA | F-16sIxodes; R-16sIxodes; 16s-Ixodes-Probe | 97 | [38] |