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Table 2 Summary of Ross River virus experimental infection studies included in the review with description of methods and vertebrate species used

From: The non-human reservoirs of Ross River virus: a systematic review of the evidence

Reference

Infection method

Virus detection methods

Vertebrate species tested (sample size)

Virus co-infection

RRV strain

Infection route

Vector used

Boyd et al. [35]

B94/20

Infected vector bite

Yes

Infection of a vector; magnitude and duration of viraemia (SMIC)

Brushtail possum Trichosurus vulpecula (10)

Simultaneous infection with Barmah Forest virus

Boyd & Kay [58]

B94/20

Infected vector bite

Yes

Infection of a vector; magnitude and duration of viraemia (CCID50)

Cat Felis catus (10); dog Canis lupus familiaris (10)

 

Ryan et al. [33]

B94/20

Infected vector bite

Yes

Infection of a vector; magnitude and duration of viraemia (TCID)

Grey-headed flying fox Pteropus poliocephalus (10)

 

Kay et al. [34]

Not stated

Intravenous injection and infected vector bite

Yes

Infection of a vector; magnitude and duration of viraemia (SMIC)

Horse Equus ferus (11)

Simultaneous infection with Murray Valley encephalitis

Kay et al. [27]

Not stated

Infected vector bite

Yes

Infection of a vector; magnitude and duration of viraemia (SMIC)

Agile wallaby Macropus agilis (9); grey kangaroo Macropus giganteus (3); rabbit Oryctolagus cuniculus (9); sheep Ovis aries (8); pig Sus scrofa (11); horse Equus ferus (11); cattle Bos taurus (6); chicken Gallus gallus domesticus (20); black duck Anas rubripes (3); little corella Cacatua sanguinea (12)

Simultaneous infection with Murray Valley encephalitis

Spradbrow [55]

T48

Subcutaneous infection

No

Magnitude and duration of viraemia (LD50)

Sheep Ovis aries (3); pig Sus scrofa domesticus (3)

 

Whitehead [38]

T48

Subcutaneous infection

No

Magnitude and duration of viraemia (LD50)

Rabbit Oryctolagus cuniculus (4); rat Rattus spp. (4); bandicoot Isoodon macrourus (4); marsupial mouse Antechinus spp. (6); chicken Gallus gallus domesticus (16); pigeon Columba livia domestica (5)

Simultaneous infection with Sindbis virus

  1. Abbreviations: SMIC, suckling mouse intracerebral injection; CCID50, cell culture infectious dose; TCID, tissue culture infectious dose; LD50, lethal dose per gm of whole blood