Fig. 3
From: Analysis of microRNA profile of Anopheles sinensis by deep sequencing and bioinformatic approaches
Validation of the selected known and novel miRNAs by quantitative real-time PCR. a Seven miRNAs (five miRNAs from the sequencing result and two miRNAs from the bioinformatic approach) were identified by qRT-PCR. b The relative expression of five miRNAs from the sequencing result showed similar expression pattern when confirmed by qRT-PCR. The transcript levels of both known and novel miRNAs were calculated relative to the amount of U6 small nuclear after normalization. The real time PCR data with bars represent the mean ± SD from three independent experiments