Table 1 Primers and protocols used for the amplification of feline hemoplasmas and housekeeping GAPDH gene control
From: Epidemiological study of hemotropic mycoplasmas (hemoplasmas) in cats from central Spain
Target gene | Product size (bp) | PCR primers (5′–3′) | PCR conditions |
|---|---|---|---|
16S rRNA hemoplasmas [8] | 170 / 193 | F: ACGAAAGTCTGATGGAGCAATA | 94 °C, 1 min; 45 cycles [94 °C, 1 min; 65 °C, 1 min; 72 °C, 30 s]; 72 °C, 10 min |
R: ACGCCCAATAAATCCGRATAAT | |||
CMt [11] | 138 | F: AGAGGCGAAGGCGAAAACT | 95 °C, 2 min; 30 cycles [95 °C, 10 s; 58 °C, 30 s; 72 °C, 30 s]; 72 °C 5 min |
R: CTACAACGCCGAAACACAAA | |||
GAPDH-Housekeeping [22] | 282 | F: CCTTCATTGACCTCAACTACAT | 95 °C, 1 min; 45 cycles [94 °C, 4 s; 57 °C, 4 s; 72 °C, 3 s]; 72 °C, 1 min |
R: CCAAAGTTGTCATGGATGACC |