Fig. 3

DGGE profiles of bacterial communities of Ae. aegypti larvae depending on their Bti tolerance level. Five representative individuals are shown per category. Each larva was labeled with a “T number” corresponding to the time point at which it died (see Fig. 1). It was followed by a second number that gives the replicate number whenever several larvae died at the same time point. Four gels were performed to analyze a total of 15, 18, 15, and 20 individual larvae from the “Control”, “Susceptible”, “Intermediate” and “Tolerant” groups, respectively (Additional file 5: Table S3). St. 1 kb + ladder was used as an external gel migration control. Several bands were excised and sequenced to identify to which bacterial genus they were affiliated (Additional file 1: Figure S1). The two bands corresponding to Bti are indicated by a red rectangle (Additional file 1: Figure S1)