Skip to main content
Fig. 2 | Parasites & Vectors

Fig. 2

From: A recombinant Fasciola gigantica 14-3-3 epsilon protein (rFg14-3-3e) modulates various functions of goat peripheral blood mononuclear cells

Fig. 2

SDS-PAGE and Western blotting analyses of the extracted rFg14-3-3e protein from the culture supernatant of P. pastoris. a Proteins were resolved on 12% acrylamide gels and stained with Coomassie brilliant blue R250. Lane M: protein molecular weight marker in kDa; Lane P: purified recombinant protein, rFg14-3-3e, which appeared as a single band of approximately 29 kDa (arrow). b The protein of interest was run under non-reducing conditions, and visualized by immunodetection using specific antibodies and enhanced chemiluminescence. Lane M: protein molecular weight marker in kDa; Lane P: loaded with recombinant protein extracted from P. pastoris. Serum from F. gigantica-infected goats detected a single band of approximately 29 kDa (arrow); Lane C1: loaded with recombinant protein extracted from P. pastoris that did not react with the serum of normal, pre-immunized, goats; Lane C2: loaded with protein extracted from the supernatant of P. pastoris cells transformed with the pPIC9K vector without Fg14-3-3e gene insert, which did not react with goat serum containing anti-F. gigantica IgG antibodies

Back to article page