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Fig. 3 | Parasites & Vectors

Fig. 3

From: A recombinant Fasciola gigantica 14-3-3 epsilon protein (rFg14-3-3e) modulates various functions of goat peripheral blood mononuclear cells

Fig. 3

Fasciola gigantica-derived rFg14-3-3e protein binds to the surface of goat PBMCs. Visualization of rFg14-3-3e protein attachment to goat PBMCs surface was carried out by incubation of goat PBMCs treated or untreated with rFg14-3-3e protein with rat anti-rFg14-3-3e primary antibody. Hoechst (blue) and Cy3-conjugated secondary antibody (red) were used to stain host cell nuclei and rFg14-3-3e protein, respectively. Surface staining was detected in rFg14-3-3e-treated cells. No staining was detectable in untreated cells. Scale-bars: 10 μm

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