Fig. 1
From: Development and validation of a duplex real-time PCR assay for the diagnosis of equine piroplasmosis
Optimization of the DNA extraction procedure. Individual Cq values with median after the duplex qPCR amplification of DNA extracted from T. equi-infected equine blood according to the standard protocol provided with the DNA extraction kit or with modifications that involved preliminary lysis of erythrocytes with RBC Lysis Solution or additional wash of the spin column containing adsorbed DNA with either Buffer AW1 (AW1 Plus) or Buffer AW2 (AW2 Plus). These samples were amplified either with or without BSA in the reaction mixture. Graph was produced using Prism 6