Fig. 4
From: Development and validation of a duplex real-time PCR assay for the diagnosis of equine piroplasmosis
Frequency distribution of Cq values obtained by testing blood samples from 430 Brazilian horses for T. equi by the duplex qPCR (a) and the single-target T. equi 18S rRNA gene-specific qPCR (b), as well as for B. caballi by the duplex qPCR (c) and the single-target B. caballi 18S rRNA gene-specific qPCR (d). For samples with amplification plots produced in both duplicates, mean of these Cq values was used for the analysis, whereas when only one of the duplicates amplified, that single Cq value was used unaltered. Graphs were generated using Prism 6