Fig. 1

PCR evaluation of subtractive hybridization efficiency. a Gel electrophoresis of subtractive hybridization products after two rounds of PCR amplification. Lane M: Ф X174-Hae III digest Marker; Lane 1: double PCR product of subtractive hybridization group; Lane 2: double PCR product of non-subtractive hybridization group; Lane 3: positive control; Lane 4: negative control; Lane 5: double PCR product of mouse control (subtractive hybridization); Lane 6: double PCR product of mouse control (no subtractive hybridization). b Gel electrophoresis of G3PDH gene PCR products using subtractive and non-subtractive cDNA as templates with 18, 23, 28 and 33 cycles of amplification