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Fig. 2 | Parasites & Vectors

Fig. 2

From: Bacillus subtilis spore with surface display of paramyosin from Clonorchis sinensis potentializes a promising oral vaccine candidate

Fig. 2

Construction of recombinant PEB03-CotC-CsPmy plasmid and preparation of rCsPmy and antibody titer analysis. a Schematic representation of genetic engineering using PEB03-CotC. b The full-length coding sequence of CsPmy was cloned into a PEB03-CotC plasmid and was confirmed by PCR with recombinant PEB03-CotC-CsPmy plasmid as template. Lane M: DNA markers; Lane 1: PEB03-CotC plasmid; Lane 2: recombinant PEB03-CotC-CsPmy plasmid; Lane 3: CotC gene. Lane 4: CsPmy gene; Lane 5: CotC-CsPmy gene. c SDS-PAGE analysis of purified recombinant CsPmy. Abbreviations: M, protein marker; CsPmy, recombinant CsPmy protein. d The antibody titer of IgG in serum of CsPmy immunized rat

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Parasites & Vectors

ISSN: 1756-3305

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