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Fig. 4 | Parasites & Vectors

Fig. 4

From: Identification, characterization and heparin binding capacity of a spore-wall, virulence protein from the shrimp microsporidian, Enterocytozoon hepatopenaei (EHP)

Fig. 4

Expression, purification and Western blot analysis of recombinant EhSWP1. a SDS-PAGE gel compared between uninduced E. coli BL21 star(DE3) cells and induced E. coli cells with 0.4 mM IPTG. b SDS-PAGE gel showing purified recombinant EhSWP1 obtained using Ni2+-NTA affinity chromatography. Lane FT shows the flow-through fraction; W1 and W5 are wash fractions 1 and 5, respectively; E1-E5 are elution fractions 1–5. c Immunoblot of recombinant EhSWP1 probed with rabbit anti-SWP1 antibody and naïve rabbit serum as a negative control. The recombinant EhSWP1 band is indicated by a black arrow. Lane M: protein molecular weight marker

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