Fig. 9

Heparin competition assay. a SDS-PAGE gel showing input recombinant EhSWP1 (black arrow) with different concentrations of soluble porcine heparin. b SDS-PAGE gel shows elution fractions after 1 h competition and revealing that binding of EhSWP1 (black arrow) to heparin beads was blocked at 100 mg/ml. c Bar graph showing percentage of heparin binding quantified from the protein bands from 3 with replicates gels (Additional file 2: Figure S2). Error bars indicate the mean ± SEM. Level of heparin binding at 0 mg/ml was used for normalization. *P ≤ 0.01; **P ≤ 0.001; ***P ≤ 0.0001