Table 1 Primer sequences, sizes of PCR amplicons and annealing temperature of the amplification of pfap2mu and pfubp1 genes
Gene | Primers (5'–3') | Size of PCR amplicon (bp) | Annealing temperature (°C) |
|---|---|---|---|
pfap2mu | Primary amplification | 2247 | 50 |
Forward: AAGACTGTCAAATGTAAAAGACCC | |||
Reverse: CTCATGTAAAACAAAAAGTGAGG | |||
Secondary amplification | 841 | 52 | |
Forward: GATATCCACAAACATTAGAAGTG | |||
Reverse: CCATCTGGTGGTGTGAAGG | |||
pfubp1 | Primary amplification | 484 | 52 |
Forward: CGCCCGTACTATGAAGAAGATC | |||
Reverse: GGCTTTTACCTGAACTGTTCAGG | |||
Secondary amplification | 304 | 57 | |
Forward: CGTAAACAGAATATTCAGGATTGC | |||
Reverse: CTAGCCCTTTATTATCATTATCG |