Table 2 PCR amplification efficiency of DNA from Enterocytozoon bieneusi ITS genotypes J and BEB4 at the MLST loci
From: Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves
ITS genotype | Farm ID | No. of specimens | No. of specimens amplified/No. of specimens analyzed | |||
|---|---|---|---|---|---|---|
MS1 | MS3 | MS4 | MS7 | |||
J | 1 | 0 | – | – | – | – |
2 | 0 | – | – | – | – | |
3 | 129 | 35/38 | 85/129 | 35/38 | 14/38 | |
4 | 15 | 14/15 | 0/15 | 14/15 | 6/15 | |
5 | 1 | – | 0/1 | – | – | |
BEB4 | 1 | 32 | 13/15 | 31/32 | 14/15 | 6/15 |
2 | 1 | 1/1 | 1/1 | 1/1 | 1/1 | |
3 | 1 | – | 0/1 | – | – | |
4 | 0 | – | – | – | – | |
5 | 25 | 15/15 | 25/25 | 15/15 | 8/15 | |
Total | Â | 204 | 78/84 | 142/204 | 79/84 | 35/84 |