Table 1 Primer sequences and locus characteristics
Locus | Accession no. | Repeat | Fragment size (bp) | Forward (5'-3')a | Reverse (5'-3') | Tm (°C)b | Genec |
|---|---|---|---|---|---|---|---|
PPEST 2 | FG10856.1 | (GCA)13 | 141 | D2-TGTCAATAGTGGCTCAATGCTC | ATTAGTCGTTTATCCTTCCCCG | 60 | PPAI004876-PA |
PPEST 10 | FG117371.1 | (TC)12 | 236 | D2-ACTGAATCTTCTGCTTTCTCCATTC | TAAGGGAAGGGGCGGAAC | 60 | PPAI001073-PA |
PPEST 11 | ES347986.1 | (GA)11 | 162 | D4-GGTGGATACTTGTGACGACTGA | CCACTCAAACTAAACTGGAAAGC | 60 | PPAI005664-PA |
PPEST 17 | FG116712.1 | (TGC)9 | 208 | D4-CTGTTCAGCAAAACGAGACG | TCCCAAGTACAAAGACGGAACT | 60 | PPAI008660-PA |
PPEST 33 | FG115100.1 | (GAA)15 | 251 | D4-ATACTCCCTCAGAACTAGCCCC | TTCGTCTTCTTCTTCTTCCTCC | 60 | PPAI005234-PA |
PPEST 34 | EY215687.1 | (AAAG)5 | 137 | D3-CACCTACAGAGATGCTGGATTG | GGGCTAAAATGTGTCTTGACTTG | 62 | PPAI003756-PA |
PPEST 40 | FG114532.1 | (AG)5 | 322 | D2-TCCCAAGGCTATTAAGTCTGGT | GGCTATCGTGCAATTTTCTTCT | 62 | PPAI005295-PA |
PPEST43 | EX474024.1 | (CT)8 | 228 | D3-AAAAGAGATTTTCGGGGAAGG | GATTGTTGAAGGAGTGAAAGGG | 62 | PPAI008911-PA |
PPEST49 | FG114549.1 | (AG)10 | 171 | D2-AAACAGCTTCAATCGCTCTGAC | CTCACTCACTCTCCCTTCGTCT | 60 | PPAI001715-PA |
PPEST68B | ES349040.1 | (CA)6 | 195 | D3-TGATTTCACCCTTGTGTTTCC | TGTGGCAACTTATTTACATCCC | 57.1 | PPAI009554-PA |
PPEST73 | EY213549.1 | (GA)7 | 244 | D3-CCAACAATCTCCTCTCTATCGC | CCCTCAAGCTAACAAACACACA | 57.1 | PPAI009131-PA |
PPEST75 | EY203801.1 | (GTT)5 | 237 | D4-TTGTCAGGAAAGGAGTTGTTCA | GTATGCAGCTCTCCCAGAAGAT | 55.5 | PPAI006509-PA |
PPEST77 | EY204214.1 | (GA)8 | 181 | D2-AATCTCAATCTGGGCAATGTGT | ACCTTCTCTGTAAATTCCCTTGG | 57.1 | PPAI004565-PA |
PPEST85 | EY208767.1 | (GA)9 | 244 | D4-GTTGAAGGGAAATTGTGAGGAT | ATGGACATTTGTGGACTCTGC | 60 | PPAI003566-PA |