First identification and multilocus genotyping of Giardia duodenalis in pet chipmunks (Eutamias asiaticus) in Sichuan Province, southwestern China

Table 1 Primer sequences, annealing temperatures and the fragment lengths of the genes used in this study

Gene	Primer	Sequence (5'-3')	Annealing temperature (°C)	Fragment length (bp)	Reference
bg	F1	AAGCCCGACGACCTCACCCGCAGTGC	65	530	[21]
	R1	GAGGCCGCCCTGGATCTTCGAGACGAC	65
	F2	GAGGCCGCCCTGGATCTTCGAGACGAC	65
	R2	CATAACGACGCCATCGCGGCTCTCAGGAA	65
tpi	F1	AAATIATGCCTGCTCGTCG	50	530	[21]
	R1	CAAACCTTITCCGCAAACC	50
	F2	CCCTTCATCGGIGGTAACTT	50
	R2	GTGGCCACCACICCCGTGCC	50
gdh	F1	TTCCGTRTYCAGTACAACTC	50	511	[21]
	R1	ACCTCGTTCTGRGTGGCGCA	50
	F2	ATGACYGAGCTYCAGAGGCACGT	50
	R2	GTGGCGCARGGCATGATGCA	50

ISSN: 1756-3305