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Fig. 4 | Parasites & Vectors

Fig. 4

From: A novel Babesia orientalis 135-kilodalton spherical body protein like: identification of its secretion into cytoplasm of infected erythrocytes

Fig. 4

a Western blot analysis of BoSBP3-like immunogenicity. Lane M: molecular weight marker; Lane 1, Lane 2 and Lane 3: reaction of rBoSBP3-like-1/-2/-3 with the serum of B. orientalis-infected buffalo; Lane 4, Lane 5 and Lane 6: reaction of rBoSBP3-like-1/-2/-3 with the serum of normal buffalo. b Identification of native BoSBP3-like in B. orientalis merozoite lysate. Lane M: molecular weight marker; Lane 1: lysate of B. orientalis-infected buffalo erythrocytes probed with serum from a rabbit immunized with rBoSBP3-like-1; Lane 2: lysate of uninfected buffalo erythrocytes probed with serum from a rabbit immunized with rBoSBP3-like-1; Lane 3: lysate of B. orientalis-infected buffalo erythrocytes probed with serum of a naïve rabbit; Lane 4: lysate of uninfected buffalo erythrocytes probed with serum of a naïve rabbit. The corresponding bands are indicated by arrows

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