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Table 1 Results of serological and molecular diagnostic tests for anaplasmosis on the six Rhodesian ridgeback dogs performed at multiple time points throughout their lives

From: A lifelong study of a pack Rhodesian ridgeback dogs reveals subclinical and clinical tick-borne Anaplasma phagocytophilum infections with possible reinfection or persistence

Clinic PCR Serology
Dog (ID) Birth- Death Lethality VMDC RIVM 2001 RIVM 2003 RIVM 2014 Total IFT Immunoblot Snap test Total
    16S rRNA 16S rRNA 16S rRNA msp2 & groEL   Whole cell P44 P44  
M12 1994–2001 Splenitis, fever and pain   1/1   1/3, spleen neg 2/3 1/2 2/2 0/4 2/5
L11 1998–2009 Malignant tumor in spleen, lung 1/4 2/3 2/9 3/4, spleen pos 5/16 2/3 3/12 3/18 7/21
L12 1998–2005 Splenitis, fever and pain   0/1 0/1 0/1, spleen neg 0/4 nd 0/3 3/5 3/7
L13 1998–2009 Haemangiosarcoma   0/1 0/1 0/2, spleen neg 0/5 nd 0/2 1/6 1/6
L21 1999–2005 Lymphoma   0/1 0/1 0/1, spleen neg 0/3 nd 1/2 0/6 1/7
N00 1995–2009 Metastasis mammae tumor   0/1 0/1 0/1, spleen nd 0/3 nd 1/1 2/6 3/6
  1. Notes: Different laboratories were involved during the years, employing varying serological and molecular tests. Results are presented separately and overall: (i) The Veterinary Microbiological Diagnostic Center, Veterinary Faculty, Utrecht University, The Netherlands (VMDC) performed serological and molecular tests for ehrlichiae and Babesia as of 2001. Immunofluorescent tests for E. phagocytophilum and E. canis were performed on the 2001 samples; (ii) The National Institute of Public Health and Environment (RIVM) employed an immunoblot with the p44 protein of A. phagocytophilum and amplified the 16S rRNA gene and confirmed the result with RLB (see Methods); (iii) The RIVM followed the same methods in 2003 with another team of technicians; (iv) The molecular detection by the RIVM in 2014 targeted a different set of genes (than in 2 and 3) in blood and spleen tissues (see Methods). The in-house SNAP® 4 Dx test (IDEXX) as serological assay was also employed.
  2. Abbreviations: neg, negative, nd not determined, pos positive