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Table 2 Clinical episodes of dog L11 in 2001 and 2002 as determined by symptoms, hematology, serology and pathogen detection by PCR or microscopy. Four clinical episodes were detected in the years 2001, 2002, 2005 and 2008. Symptoms were not always accompanied by fever and bleeding tendency was not always apparent. Other symptoms as lymphadenopathy, muscle pain, lameness, reluctance to move and lethargy were also observed

From: A lifelong study of a pack Rhodesian ridgeback dogs reveals subclinical and clinical tick-borne Anaplasma phagocytophilum infections with possible reinfection or persistence

Year 2001 2002
Day/Month 23/04 27/04 14/05 29/06 28/08 09/02 07/03 21/06 03/09 23/09 25/09 18/10
Hematology (reference)
 Erythrocytes (> 5.5/pl) 7.05 6.21 6.78 7.84 7.56 7.11   6.75 7.15 5.72 6.33 6.46
 Hemoglobin (> 8.8 mmol/l) 10.66 9.42 10.23 11.90 10.97 10.60   10.66 11.04 8.37 9.05 9.61
 HT (> 42%) 51 46 48 61 56 54   49 52 39 43 50
 MCV (65.5–75.5 fl) 71.70 73.60 71.10 77.30 73.90 75.40   73.20 72.50 67.80 68.20 78.10
 MCH (0.90–1.55 pmol) 1.46 1.47 1.46 1.47 1.40 1.45   1.53 1.49 1.42 1.39 1.45
 MCHC (20.9–22.3 mmol/l) 21.08 20.58 21.2 19.59 19.59 19.78   21.58 21.20 21.64 21.02 19.10
 Thrombocytes (150–400/nl) 9 50 131 23 17 192   216 180 50 187 206
 Leukocytes (5.9–13.8/nl) 11.4 11.6 14.5 8.4 8.9 7.9   7.9 8.2 7.8 6.8 8.5
 Neutrophils (55–75%) 73 79 71 55 67 65   66 61 78 42 73
 Lymphocytes (13–30%) 16 18 16 35 22 27   23 33 9 52 21
 Monocytes (< 5%) 8 1 4 9 5 6   2 5 9 4 5
 Eosinophils (< 4%) 2 1 6 1 4 1   9 1 0 2 1
 Basophils (< 1%) 0   0 0 0 0   0 0 0 0 0
 Staphylococcus (< 4%) 1 1 3 0 2 1   0 0 4 0 0
Serology
Anaplasma Immunoblot neg2 neg2 neg2 neg3 neg3 neg3   neg3 neg3 pos 3 pos 3 pos 3
IFT pos 1 pos 1 neg1          
Snap test   neg neg neg neg    neg neg   pos neg
Borrelia WHC-titer   80 40          
C6 Snap test neg neg neg neg neg    neg neg   neg neg
Coagulation test             normal
 Microscopy (morulae) pos neg neg neg neg neg    neg pos neg neg
 PCR - DNA pos 2,4 neg2 pos 2 neg2 neg3 neg3   neg3 neg3,4 pos 1,3,4 pos 3 neg1,3
 RLB A. phago variant 1 pos 2   neg        pos 3 neg  
A. phago variant 2 neg   neg        neg neg  
A. phago variant 3 neg   neg        neg neg  
A. phago variant 4 neg   pos 2        pos 3 neg  
E. equi neg   neg          
HGE agent neg   neg        pos 3 pos 3  
 Clinics
  Body temperature 38.8 38.4 38.4 38.5 38.2 38.3 38.3 38.2   39.6   38.5
Bleeding ecchm resolv no no no no no no no no no no
Other listless, lymphaden. R popliteus pseudolac   lame myositis, lays, pseudolac, lameness pseudolac dyspneu cough
Therapy   doxy    doxy   doxy doxy    doxy amox/clv
Ticks on dog yes      yes   yes yes yes   yes
  1. Notes: Deviant or positive laboratory results are shown in bold. Serology and Anaplasma DNA detection employed different methods which were performed in different years and laboratories indicated by numbers 1 to 4. Assay method depended on laboratory and year of processing and is indicated as:1VMDC till 2008; 2RIVM in 2001; 3RIVM in 2003; and 4RIVM in 2014. The in-house SNAP® 4Dx test (IDEXX) was employed to determine antibodies against the p44 antigen, which was also detected by immunoblot in the first 2 years as was the IFA (immunofluorescent assay) in the first year. The agent detection by 16S rRNA gene amplification and confirmed by reverse line blot hybridization (RLB) detecting variants of A. phagocytophilum was performed in the first two years. Time of doxycycline therapy and of tick collections from skin are notated
  2. Abbreviations: pos positive, neg negative, pseudolac pseudo-lactation after estrous, OVH spaying by ovariohysterectomym lymphaden lymphadenopathy, ecchm ecchymosis, resolv resolving, A. phago A. phagocytophilum, E. equi Ehrlichia equi, doxy doxycyclin, amox/clv amoxicillin with clavulanic acid