Skip to main content

Table 3 Clinical episodes of dog L11 from 2003 to 2009 as determined by symptoms, hematology, serology and pathogen detection by PCR or microscopy. Symptoms were not always accompanied by fever and bleeding tendency was not always apparent. Other symptoms as lymphadenopathy, muscle pain, lameness, reluctance to move and lethargy were also observed

From: A lifelong study of a pack Rhodesian ridgeback dogs reveals subclinical and clinical tick-borne Anaplasma phagocytophilum infections with possible reinfection or persistence

Year 2003 2004 2005 2007 2008 2009
Day/Month 18/08 09/10 19/04 29/04 09/03 29/11 27/09 17/10 22/02 11/09 12/05
Hematology (reference)
 Erythrocytes (> 5.5/pl)   acanthosis 7.5   7.5 acanthosis normal   normal normal  
 Hemoglobin (> 8.8 mmol/l)    11.4   11,4       
 HT (> 42%)    52 80 57   60   50   
 MCV (65.5–75.5 fl)    69.5         
 MCH (0.90–1.55 pmol)    1.51         
 MCHC (20.9–22.3 mmol/l)    21.8         
 Thrombocytes (150–400/nl) >>> < 201   236 < >>>   <<< <  
 Leukocytes (5.9–13.8/nl) 16 7 6.9   14.5 6 10   30 60  
 Neutrophils (55–75%) 87 60 69   80 83 58   80 85  
 Lymphocytes (13–30%) 9 25 23   7 11 36   15 10  
 Monocytes (< 5%) 4 12 4   13   4   4 5  
 Eosinophils (< 4%) 1 3 4   0 1 2   1   
 Basophils (< 1%)    0   0 4 0     
 Staphylococcus (< 4%)    0   0   0     
Serology
Anaplasma Immunoblot   neg          
IFT            
Snap test neg neg neg neg neg pos neg neg pos neg  
Borrelia WHC-titer       80      
C6 Snap test neg neg neg neg neg neg neg neg neg neg  
 Coagulation/other tests   acanthosis    RF neg Coombs + RF neg     hypergamma proteinaemia
 Microscopy (morulae) neg neg   neg neg neg neg   pos neg  
PCR - DNA neg3 neg1,3    neg1     neg1   spleen pos 4
 RLB A. phago variant 1            
A. phago variant 2            
A. phago variant 3            
A. phago variant 4            
E. equi            
HGE agent            
 Clinics
  Body temperature 38.6   38.9 39.6 39.3   38.9   39.4   
Bleeding no no no no no petechiae no no erythema belly  
Other    Pseudolac heart after OVH non-healing wound   arthrosis in shoulders dyspneu lick granuloma euthanasia
Therapy     doxy doxy      pleiomorphic malignant lung tumour
Ticks on dog   yes   yes   yes yes     
  1. Notes: Deviant or positive laboratory results are shown in bold. Serology and Anaplasma DNA detection employed different methods which were performed in different years and laboratories indicated by numbers 1 to 4. Assay method depended on laboratory and year of processing and is indicated as:1VMDC till 2008; 2RIVM in 2001; 3RIVM in 2003; and 4RIVM in 2014. The in-house SNAP® 4Dx test (IDEXX) was employed to determine antibodies against the p44 antigen, which was also detected by immunoblot in the first 2 years as was the IFA (immunofluorescent assay) in the first year. The agent detection by 16S rRNA gene amplification and confirmed by reverse line blot hybridization (RLB) detecting variants of A. phagocytophilum was performed in the first two years. Time of doxycycline therapy and of tick collections from skin are notated
  2. Abbreviations: pos positive, neg negative, pseudolac pseudo-lactation after estrous, OVH spaying by ovariohysterectomy, lymphaden lymphadenopathy, ecchm ecchymosis, resolv resolving, A. phago A. phagocytophilum, E. equi Ehrlichia equi, doxy doxycyclin