Fig. 2

C.sp_P exposure causes adult mortality but does not influence fecundity of surviving females. Female An. gambiae mosquitoes were fed a 10⁷ CFU/ml dose of either C.sp_P or Pantoea sp. (P. sp.) planktonic culture or an equal volume of 1× PBS in a 1.5% sucrose meal for 24 h. Experimental sugar meals were then removed, and all treatments were provided 10% sucrose ad libitum. Females were blood-fed on the third day post-exposure and allowed to oviposit for 2 days. Females that did not blood-feed were removed from the experiment. Data for each treatment were collected over 3–4 biological replicates. a Exposure to C.sp_P 10⁷ CFU/ml significantly decreased survival relative to PBS, while exposure to Pantoea sp. did not have a significant effect on survival (PBS vs C.sp_P 10⁷, χ² = 98.7, df = 1, P < 0.0001; PBS vs Pantoea 10⁷, χ² = 0.1, df = 1, P = 0.77). Total sample sizes for a: n_PBS = 283, n_{Pantoea 10^7} = 162, n_{C.sp_P 10^7} = 264. b, c Treatment did not significantly affect the proportion of females that oviposited (b, χ² = 0.68, df = 2, P = 0.7116) nor their fecundity (c, F_{(2, 138)} = 1.165, df = 2, P = 0.1474). Total sample sizes for b and c: n_PBS = 56, n_{Pantoea 10^7} = 35, n_{C.sp_P 10^7} = 50. Data were analyzed using pairwise Log-Rank tests in a, Kruskal-Wallis test in b and a one-way ANOVA in c. Black bars represent the median value in (b) and (c)