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Fig. 2 | Parasites & Vectors

Fig. 2

From: Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids

Fig. 2

Specificity of the PKD-RPA assay. Specific amplification of T. bryosalmonae DNA only is indicated by a color signal on both control and test lines for T. bryosalmonae positive samples only, and not with other tested DNA (which show only one color signal, on the control line). C-line: control signal; T-line: location of T. bryosalmonae amplicon; Lane T. bryosalmonae: DNA from pure Tetracapsuloides bryosalmonae mature spore sacs; Lane Inf. F. sultana, DNA from T. bryosalmonae infected Fredericella sultana colonies; Lane Inf. fish tissues: DNA from T. bryosalmonae infected fish kidney; Lane B. plumatellae: DNA from confirmed Buddenbrockia plumatellae sample; Lane M. cerebralis: DNA from Myxobolus cerebralis spores. Lane SPF F. sultana: DNA from specific pathogen-free Fredericella sultana colonies; Lane SPF fish tissues: DNA from specific pathogen-free fish kidney; Lane No-template control: DNA was omitted from the reaction

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