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Fig. 3 | Parasites & Vectors

Fig. 3

From: Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids

Fig. 3

Sensitivity of the PKD-RPA assay compared with the PCR assay. The figure shows the lower detection limit of the developed PKD-RPA assay compared with the diagnostic PCR assay. Ten-fold serial dilutions of pure DNA from T. bryosalmonae spore sac (10 ng) was used for this purpose. The PKD-PCR and PKD-RPA assays could detect down to 1 pg and 100 fg of T. bryosalmonae DNA, respectively, which is indicated by the presence of a PCR band at 435 bp and the presence of two color signals on the LFD, respectively. C-line: control signal; T-line: location of PK -amplicon; Lane Mar: 100 bp DNA ladder (Invitrogen); Lane NTC: No-template control

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