Fig. 3From: Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonidsSensitivity of the PKD-RPA assay compared with the PCR assay. The figure shows the lower detection limit of the developed PKD-RPA assay compared with the diagnostic PCR assay. Ten-fold serial dilutions of pure DNA from T. bryosalmonae spore sac (10 ng) was used for this purpose. The PKD-PCR and PKD-RPA assays could detect down to 1 pg and 100 fg of T. bryosalmonae DNA, respectively, which is indicated by the presence of a PCR band at 435Â bp and the presence of two color signals on the LFD, respectively. C-line: control signal; T-line: location of PK -amplicon; Lane Mar: 100 bp DNA ladder (Invitrogen); Lane NTC: No-template controlBack to article page