Evaluation of fluorimetry and direct visualization to interpret results of a loop-mediated isothermal amplification kit to detect Leishmania DNA

Table 2 Results obtained with the different instruments in each of the different experiments proposed. Discordant results are shown in bold letters. Sample order in the table is the same as in LAMP analyses

Sample ID/ patient type	Sample type	LnPCR	qPCR	LAMP results by instrument/DNA preparation
				LF-160 /B&S	LF-160 /QIA	Genie III® /B&S	Genie III® /QIA	ESEQuant TS2.2/B&S	ESEQuant TS2.2/QIA
VL1/C	Peripheral blood	Pos	Pos	Pos	Pos	Pos	Pos	Pos	Pos
VL2/C	Bone marrow aspirate	Pos	Pos	Pos	Pos	Pos	Pos	Pos	Pos
VL3/C	Peripheral blood	Pos	Pos	Pos	Pos	Pos	Pos	Pos	Pos
VL4/C	Peripheral blood	Pos	Pos	Pos	Pos	Pos	Pos	Pos	Pos
VL5/NC	Bone marrow aspirate	Neg	Neg	Neg	Neg	Neg	Neg	Neg	Neg
VL6/C	Bone marrow aspirate	Pos	Pos	Unc	Pos	Pos	Pos	Pos	Pos
VL7/NC	Peripheral blood	Neg	Neg	Pos	Neg	Neg	Neg	Neg	Neg
VL8/NC	Bone marrow aspirate	Neg	Neg	Pos	Neg	Neg	Neg	Neg	Neg
VL9/C	Bone marrow aspirate	Pos	Pos	Pos	Pos	Pos	Pos	Pos	Pos
VL10/NC	Peripheral blood	Neg	Neg	Neg	Neg	Neg	Neg	Neg	Neg

Abbreviations: ID Identification number in this study, Pos Positive result, Neg negative result, C confirmed VL case, NC non-confirmed VL case, Unc uncertain result

ISSN: 1756-3305