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Fig. 5 | Parasites & Vectors

Fig. 5

From: Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella

Fig. 5

Interaction between EtCDPK4 and EtSerpin assessed with Co-IP. a DF-1 cells were transfected with pCDNA3.1-flag-EtCDPK4 and pCAGGS-EtSerpin and cellular lysates were analyzed with immunoblotting with antisera against EtCDPK4 and EtSerpin. b The Co-IP was performed using immobilised antisera against EtCDPK4. Detection of eluted proteins on immunoblots was by EtSerpin and/or anti-flag for EtCDPK4 antibodies

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