Essential oils of Citrus aurantifolia , Anthemis nobile and Lavandula officinalis : in vitro anthelmintic activities against Haemonchus contortus

Table 2 Effects (mean percentage ± SE) of tested essential oils on inhibition of egg hatching of H. contortus

Concentration (mg/ml)	C. aurantifolia	A. nobilis	L. officinalis
50	100.0 ± 0.0^a,b	99.0 ± 0.38^a,b	100.0 ± 0.0^a,b
25	100.0 ± 0.0^a,b	99.4 ± 0.87^a,b	100.0 ± 0.0^a,b
12.5	98.9 ± 1.87^a,b	97.0 ± 2.38^a,b	99.8 ± 0.30^a,b
6.25	96.4 ± 2.61^a,b	95.7 ± 2.13^a,b	99.4 ± 0.94^a,b
3.125	95.9 ± 2.41^a,b	86.4 ± 4.71^b	92.3 ± 3.03^b
1.562	86.9 ± 2.33^b	74.5 ± 3.58^b	82.8 ± 3.62^b
0.781	56.2 ± 3.97^b	63.3 ± 4.12^b	85.0 ± 3.15^b
0.390	42.1 ± 4.70^b	26.6 ± 1.46^b	81.8 ± 1.36^b
0.195	14.9 ± 3.89^b	13.4 ± 2.33^b	9.5 ± 1.54^b
0.097	13.0 ± 1.44^b	14.1 ± 2.60^b	6.8 ± 1.69
	IC₅₀ = 0.694 mg/ml	IC₅₀ = 0.842 mg/ml	IC₅₀ = 0.316 mg/ml
	R² = 0.985	R² = 0.978	R² = 0.950
Thiabendazol (0.025 mg/ml)	100.0 ± 0.0^b	100.0 ± 0.0^b	100.0 ± 0.0^b
Tween 80 (3%)	7.6 ± 0.85	7.4 ± 3.94	3.5 ± 0.82

Abbreviations: IC₅₀, inhibitory concentration 50%; R², nonlinear correlation coefficient
^aNo statistically significant difference between positive control and treatments
^bSignificant difference between positive control or treatments with negative control (ANOVA, followed by Tukey test; P < 0.05)