Fig. 2

Effect of serum/protein supplements in various basic media with regards to the mean values of T₉₀ of L. loa L3. Infective larvae of Loa loa were cultured with three different media (RPMI, DMEM and IMDM) with each of the four serum/protein supplements [Albumax (a), FBS (b), BSA (c) and NCS (d)] without feeder cells. Pairwise multiple comparisons: Dunn’s post-hoc test for multiple comparisons of independent samples. The P-values indicated here are those that were found significant when comparing two concentrations of the serum/protein. Number of observations: n = 12. Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; IMDM, Iscove’s modified Dulbecco’s medium; RPMI, Roosevelt Memorial Park Institute; BSA, bovine serum albumin; FBS, fetal bovine serum; NCS, newborn calve serum; L3, third-stage infective larva; T₉₀, mean duration at which 90% of parasites were fully active