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Fig. 4 | Parasites & Vectors

Fig. 4

From: Evaluation of in vitro culture systems for the maintenance of microfilariae and infective larvae of Loa loa

Fig. 4

Effect of monkey kidney cells in various serum/protein supplemented basic media with regards to the mean values of T90 (days) of L. loa L3. The black arrows indicate the T90 values in absence of the feeder cells. Infective larvae of Loa loa were cultured with three different media (RPMI, DMEM and IMDM) with each of the four serum/protein supplements [Albumax (a), FBS (b), BSA (c) and NCS (d)] and (LLC-MK2) feeder cells. For each medium and each concentration of the serum/protein, culture with monkey kidney cells was compared to serum free culture (Mann-Whitney U-test). Number of observations: n = 12. Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; IMDM, Iscove’s modified Dulbecco’s medium; RPMI, Roosevelt Memorial Park Institute; BSA, bovine serum albumin; FBS, fetal bovine serum; NCS, newborn calve serum; L3, third-stage infective larva; T90, mean duration at which 90% of parasites were fully active; ¥: significantly different from the equivalent cell free culture

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