Table 1 Statistical report on the effect of serum/protein concentration in various culture systems with regards to the mean values of T₉₀ of L. loa microfilariae and L3

Loa loa mf

No

DMEM

Albumax

4.2585

3

0.2349

BSA

9.1681

3

0.0271

FBS

9.6542

3

0.0218

NCS

9.5639

3

0.0227

IMDM

Albumax

16.1960

3

0.0010

BSA

22.8860

3

< 0.0001

FBS

19.1060

3

0.0003

NCS

23.1030

3

< 0.0001

RPMI

Albumax

20.5860

3

0.0001

BSA

17.1480

3

0.0007

FBS

7.5795

3

0.0556

NCS

11.3590

3

0.0099

LLC-MK2

DMEM

Albumax

6.9561

3

0.0733

BSA

6.1987

3

0.1023

FBS

4.9629

3

0.1745

NCS

6.1198

3

0.1059

IMDM

Albumax

0.7759

3

0.8552

BSA

1.7466

3

0.6266

FBS

3.0427

3

0.3851

NCS

0.8119

3

0.8466

RPMI

Albumax

16.8520

3

0.0008

BSA

14.6670

3

0.0021

FBS

0.3286

3

0.9546

NCS

2.3611

3

0.5009

Loa loa L3

No

DMEM

Albumax

7.0250

3

0.0711

BSA

8.3441

3

0.0394

FBS

8.1322

3

0.0434

NCS

6.0725

3

0.1081

IMDM

Albumax

1.7999

3

0.6150

BSA

1.8722

3

0.5993

FBS

3.8436

3

0.2789

NCS

9.6063

3

0.0222

RPMI

Albumax

8.4292

3

0.0379

BSA

4.2135

3

0.2393

FBS

6.5292

3

0.0885

NCS

1.4905

3

0.6845

LLC-MK2

DMEM

Albumax

1.8177

3

0.6111

BSA

1.3618

3

0.7145

FBS

5.8987

3

0.1166

NCS

1.6916

3

0.6388

IMDM

Albumax

16.0890

3

0.0011

BSA

5.2627

3

0.1535

FBS

2.1496

3

0.5419

NCS

4.9566

3

0.1750

RPMI

Albumax

6.2386

3

0.1006

BSA

5.5548

3

0.1354

FBS

4.0227

3

0.2590

NCS

5.6006

3

0.1327