Fig. 2
From: Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
Confirmation of integration of pBS-EGRADE into the ef-1α locus. a Schematic diagram and results of PCR to confirm the integration of pBS-EGRADE into the ef-1α locus. PCR-1, -2 and -3 were done with primer sets Integ-F/GFP-R, hDHFR-F/Integ-R and GFP-F/hDHFR-R, respectively. b Schematic diagram and Southern blot analysis to confirm the integration of pBS-EGRADE into ef-1α locus. Two μg of samples genomic DNA were digested with Sca I and Sph I, and hybridized with 3′-ef-1α and gfp probes. Abbreviations: GI, genome-integrated; WT, wild type; C-, pBS-EGRADE plasmid control