Table 1 List of primers used in this study
From: Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
Primer | Sequence (5'–3')a |
|---|---|
Bg 5′-ef-1α-F (Hind III) | GACGGTATCGATAAGCTTCACTGTATAACGGATGAAGGT |
Bg 5′-ef-1α-R (Hind III) | CACCATGATATCAAGCTTTTTGGTAAAGGTTGACGATA |
GFP-F (EcoR V) | ATCGATAAGCTTGATATCATGGTGAGCAAGGGCGA |
GFP-R (EcoR V) | CTGCAGGAATTCGATATCTTACTTGTACAGCTCGTCCATG |
Bb 3′-rap-F (Sma I) | GAATTCCTGCAGCCCGGGGATGAGATGCGTTTATAATGGC |
Bb 3′-rap-R (Sma I) | ACTAGTGGATCCCCCGGGCCTACGAACGATATGTCAAAGAG |
Bg 5′-actin-F (Hind III) | GACGGTATCGATAAGCTTCCAGTAAAAAGTGACTACCATA |
Bg 5′-actin-R (Hind III) | AACCATGATATCAAGCTTTTTGATAACGTAATAGCTCTGTA |
hDHFR-F (EcoR V) | ATCGATAAGCTTGATATCATGGTTGGTTCGCTAAAC |
hDHFR-R (EcoR V) | CTGCAGGAATTCGATATCTTAATCATTCTTCTCATATACTTC |
Bg 3′-ef-1α-F (Sma I) | GAATTCCTGCAGCCCGGGAGCTGATTATTTCGTGTTAACT |
Bg 3′-ef-1α-R (Sma I) | ACTAGTGGATCCCCCGGGGATTGGTAGTATTTGTCGTCAT |
EGR-F (Sal I) | CCCCCCCTCGAGGTCGACCACTGTATAACGGATGAAGGT |
EGR-R (Sal I) | CTTATCGATACCGTCGACCCTACGAACGATATGTCAAAGAG |
Integ-F | TAGCAGCCAAGCGAGATA |
Integ-R | CAACTTAGATTGATCGGTG |
Probe-GFP-F | ATGGTGAGCAAGGGCGA |
Probe-GFP-R | TTACTTGTACAGCTCGTCCATG |
Probe-3′-ef-1α-F | ATCCCCTGTCTCAATGG |
Probe-3′-ef-1α-R | GATTGGTAGTATTTGTCGTCA |