Table 1 Summary of the characteristics of real-time PCR assays targeting Leishmania kDNA, in chronological order
From: Real-time PCR applications for diagnosis of leishmaniasis
Geographical region | Type of sample | Assay type | Assay chemistry | Typing | Reference |
|---|---|---|---|---|---|
Old World | Mouse tissues | Quantitative | Intercalating dye | No | [7] |
Old World | Parasite cultures | Qualitative | Intercalating dye | Yes | [96] |
Old World | Blood/ bone marrow | Quantitative | Fluorescent probe | No | [18] |
Old World | Blood/ lymph node/skin | Quantitative | Fluorescent probe | No | [65] |
Old World | Blood/ lymph node/skin | Quantitative | Fluorescent probe | No | [67] |
Old World | Blood/ bone marrow | Quantitative | Fluorescent probe | No | [80] |
Old World | Urine/ blood/ bone marrow | Quantitative | Fluorescent probe | No | [46] |
Old World | skin lesions | Qualitative | Intercalating dye | Yes | [97] |
New World | blood | Quantitative | Intercalating dye | No | [106] |
Old World | Blood/ bone marrow | Quantitative | Fluorescent probe | No | [3] |
New World | Bone marrow | Qualitative | Intercalating dye/ Fluorescent probe | No | [62] |
Old World/ New World | Blood/ skin biopsy | Quantitative | Intercalating dye/ Fluorescent probe | Yes | [20] |
New World | Sand flies | Quantitative | Intercalating dye | No | [32] |
Old World | Blood | Qualitative | Intercalating dye | No | [70] |
Old World | Ticks | Quantitative | Fluorescent probe | No | [94] |
Old World | Blood/ lymph node/ Oral swab/ Conjunctival swab | Quantitative | Fluorescent probe | No | [50] |
Old World | Blood/ ticks | Quantitative | Fluorescent probe | No | [93] |
New World | Blood/ bone marrow/ skin biopsy/ sand flies | Qualitative | Intercalating dye | Yes | [98] |
Old World | Blood/ bone marrow | Quantitative | Fluorescent probe | No | [61] |
New World | Blood/ skin biopsy | Quantitative | Intercalating dye | Yes | [59] |
New World | Skin biopsy | Quantitative | Intercalating dye | No | [13] |
Old World | Blood/ lymph nodes/ bone marrow/ conjunctival swab | Qualitative | Intercalating dye | No | [64] |
Old World | Blood/ lymph node/ dog hairs | Quantitative | Fluorescent probe | No | [63] |
New World | Blood /bone marrow/ skin biopsy/ conjunctival swab | Qualitative | Intercalating dye | No | [49] |
Old World/ New World | Blood/ conjunctival swab | Quantitative | Intercalating dye | Yes | |
New World | Sand flies | Qualitative | Intercalating dye | No | [88] |
New World | Skin biopsy/ scraping/ cytology brushes | Quantitative | Intercalating dye | No | [78] |
Old World/ New World | Liver | Quantitative | Fluorescent probe | No | [19] |
New World | Oral swabs/ conjunctival swab/ blood/ lymph node | Quantitative | Intercalating dye | No | [51] |
New World | Blood | Qualitative | Intercalating dye | Yes | [99] |
New World | Skin | Quantitative | Intercalating dye | No | [107] |
New World | Blood/ urine | Quantitative | Intercalating dye | No | [45] |
New World | Lesion swab/ biopsy | Qualitative | Intercalating dye/ Fluorescent probe | Yes | [22] |
Old World | Sand flies | Qualitative | Intercalating dye | No | [92] |
Old World/ New World | Blood/ conjunctival swab | Quantitative | Intercalating dye | Yes | [17] |
Old World | Sand flies | Quantitative | Intercalating dye | No | [87] |
New World | Parasite cultures | Quantitative | Intercalating dye | No | [30] |
New World | Blood | Quantitative | Fluorescent probe | No | [81] |
New World | Blood/ splenic aspirate/skin | Quantitative | Fluorescent probe | No | [82] |
New World | Cerebrospinal fluid/ CNS tissues/ spleen | Quantitative | Fluorescent probe | No | [108] |
New World | Lymph node/ bone marrow | Quantitative | Intercalating dye | Specific for L. infantum | [109] |