Table 2 Summary of the characteristics of real-time PCR assays targeting Leishmania rDNA, in chronological order
From: Real-time PCR applications for diagnosis of leishmaniasis
Geographical region | Type of sample | Assay type | Assay chemistry | Typing | Reference |
|---|---|---|---|---|---|
Old World | Blood | Quantitative | Fluorescent probe | No | [26] |
Old World/ New World | Blood/ bone marrow/ skin biopsy | Quantitative | Fluorescent probe | Yes | [101] |
New World | Cell cultures | Quantitative | Intercalating dye | No | [110] |
New World | Skin biopsy | Quantitative | Fluorescent probe | No | [111] |
Old World | Mouse skin biopsy | Quantitative | Fluorescent probe | No | [112] |
Old World | Bone marrow | Quantitative | Intercalating dye | No | [113] |
Old World | Smears from cutaneous lesions/ blood/ skin or spleen biopsy | Quantitative | Intercalating dye | Yes | [28] |
New World | Sand flies | Quantitative | Intercalating dye | No | [32] |
New World | Blood/skin biopsy | Quantitative | Intercalating dye | Yes | [59] |
Old World | Blood/ bone marrow/ lymph node/cutaneous lesion aspirates | Qualitative | Fluorescent probe | Yes | [71] |
Old World | Fixed lymph node biopsy | Quantitative | Fluorescent probe | Specific for L. tropica | [114] |
New World | Swab/ lesion aspirates | Quantitative | Fluorescent probe | No | [44] |
New World | Parasite cultures | Qualitative | Intercalating dye | Yes | [27] |
Old World | Blood/ bone marrow | Quantitative | Intercalating dye | Yes | [102] |
Old World | Fixed skin biopsy | Quantitative | Fluorescent probe | No | [115] |
New World | Ticks | Qualitative | Intercalating dye | No | [95] |
Old World/ New World | Blood/ skin biopsy/ bone marrow | Qualitative | Intercalating dye | Yes | [58] |
Old World | Sand flies | Qualitative | Fluorescent probe | Yes | [92] |
New World | Skin biopsy | Quantitative | Intercalating dye | No | [76] |
New World | Parasite cultures | Quantitative | Intercalating dye | No | [30] |