Echinococcus multilocularis and Echinococcus shiquicus in a small mammal community on the eastern Tibetan Plateau: host species composition, molecular prevalence, and epidemiological implications

Table 1 Primer sequences, lengths of PCR amplicons and annealing temperatures

Primers	Original code	Species	Target genes	Primer sequences	Amplicon length (bp)	Annealing temperature (°C)	Reference
CO1JP2	COIF	Taeniidae gen. sp.	cox1	TTGAATTTGCCACGTTTGAATGC	875	52	[26]
CO1JP2	COIR	Taeniidae gen. sp.	cox1	GAACCTAACGACATAACATAATGA	875	52	[26]
ND1Em	EmF19/3	E. multilocularis	nad1	TAGTTGTTGATGAAGCTTGTTG	207	53	[27]
ND1Em	EmR6/1	E. multilocularis	nad1	ATCAACCATGAAAACACATATACAAC	207	53	[27]
ND1Es	EsF50	E. shiquicus	nad1	TTATTCTCAGTCTCGTAAGGGTCCG	442	60	[27]
ND1Es	EsR73	E. shiquicus	nad1	CAATAACCAACTACATCAATAATT	442	60	[27]
ND1Eg	Eg1F81	E. granulosus	nad1	GTTTTTGGCTGCCGCCAGAAC	226	62	[27]
ND1Eg	Eg1R83	E. granulosus	nad1	AATTAATGGAAATAATAACAAACTTAATCAACAAT	226	62	[27]

ISSN: 1756-3305