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Fig. 2 | Parasites & Vectors

Fig. 2

From: Competence of mosquitoes native to the United Kingdom to support replication and transmission of Rift Valley fever virus

Fig. 2

Percentage survival of mosquitoes. a Effect of temperature on Ae. detritus survival. Mosquitos ingesting a blood meal containing at 106 PFU/mL RVFV strain ZH501, Lunyo or an uninfected control were maintained at 20 or 25 °C. Survival did not differ statistically between strains or controls at 20 °C (Log-rank (Mantel-Cox) χ2 = 1.174, df = 2, P = 0.5560) or at 25 °C (χ2 = 0.9600, df = 2, P = 0.9600) so datasets were pooled for analysis, demonstrating reduced survival at 25 °C compared to 20 °C. The effects of viral dose on survival are presented in panels b-d comparing 106 PFU/ml vs 107 PFU/ml vs uninfected controls. b Cx. pipiens f. pipiens (Caldbeck). c Cx. pipiens hybrid (Brookwood). d Ae. detritus. Survival was monitored periodically for up to 21 days. Kaplan-Meier plots were generated in GraphPad Prism, bars represent the 95% confidence interval, the numbers tested are written within parentheses (n). Statistical differences were calculated by Log-Rank test with Bonferroni-corrected thresholds, dotted lines compare strain: Lunyo, solid lines: ZH501. Demonstrating reduced survival for Cx. pipiens Caldbeck and Ae. detritus after ingestion of blood containing 107 PFU/ml compared to controls and their lower dose counterparts but no differences between doses or controls ingested by the Brookwood line mosquitoes

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