Competence of mosquitoes native to the United Kingdom to support replication and transmission of Rift Valley fever virus

Table 1 Provenance of mosquitoes used in Rift Valley fever virus vector competence studies

Species (shorthand)	Location (Reference)	GPS coordinates (DMS)	Origin	Generation	Collection method	Habitat
Cx. pipiens Caldbeck (CBK)	Caldbeck, Worcester Park, London	51°22'51.24"N, 0°14'20.399"W	Larvae, pupae (colonised)	> F100^a	^c	Freshwater pond/drainage ditch
Cx. pipiens Brookwood (BKW)	Brookwood, Surrey	51°18'23.4"N, 0°37'58.08"W	Larvae, pupae (colonised)	> F100^b	^c	Container
Cx. pipiens (sensu lato), Cx. modestus	Cliffe Pools, Kent	51°28'21.032"N, 0°28'31.575"E	Larvae, pupae	F0	Dipping	Freshwater pond/drainage ditch
Cx. pipiens (s.l.), Cx. modestus	Elmley National Nature Reserve, Kent	51°22'59.288"N, 0°46'57.459"E	Larvae, pupae	F0	Dipping	Freshwater pond/drainage ditch
Cx. pipiens (s.l.)	West Byfleet, Surrey	51°20'35.084"N, 0°29'56.664"W	Eggs	F0	Dipping	Container
Ae. detritus, Ae. caspius	Wallasea Island, Essex	51°36'55.065"N, 0°49'22.066"E	Adults	F0	CO₂ traps	Saltmarsh
Ae. rusticus, Ae. cantans/Ae. annulipes	Bartley Heath, Hampshire	51°16'32.568"N, 0°57'18.207"W	Adults	F0	Human landing	Woodland/temporary groundwater
Ae. detritus	Dee Marsh, Cheshire	53°16'39.48"N, 3°4'5.286"W	Larvae, pupae	F0	Dipping	Saltmarsh

^aThe Caldbeck colony was established from filial generation ~f50 at APHA in February 2015
^bThe Brookwood colony ~f51 in February 2016
^cCollected, colonised and characterised by the Pirbright Institute [22]
Dipping refers to the use of pans to collect larvae using a scooping action to target collection of Aedes spp. or a tilting action allowing the passive flow of water into the pan to target Culex larvae as described previously [50]. The females of Ae. cantans and Ae. annulipes cannot be reliably identified based on morphological traits [51]. Our attempts to molecularly confirm the identity of specimens from Bartley Heath by using the DNA barcoding retrieved ambiguous results for the species; therefore, we treat Ae. cantans/Ae. annulipes in our dataset. Locations are based on survey sites from [22, 52,53,54], a map of locations is presented in Additional file 1: Figure S1

ISSN: 1756-3305