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Table 1 Provenance of mosquitoes used in Rift Valley fever virus vector competence studies

From: Competence of mosquitoes native to the United Kingdom to support replication and transmission of Rift Valley fever virus

Species (shorthand) Location (Reference) GPS coordinates (DMS) Origin Generation Collection method Habitat
Cx. pipiens Caldbeck (CBK) Caldbeck, Worcester Park, London 51°22'51.24"N, 0°14'20.399"W Larvae, pupae (colonised) > F100a c Freshwater pond/drainage ditch
Cx. pipiens Brookwood (BKW) Brookwood, Surrey 51°18'23.4"N, 0°37'58.08"W Larvae, pupae (colonised) > F100b c Container
Cx. pipiens (sensu lato), Cx. modestus Cliffe Pools, Kent 51°28'21.032"N, 0°28'31.575"E Larvae, pupae F0 Dipping Freshwater pond/drainage ditch
Cx. pipiens (s.l.), Cx. modestus Elmley National Nature Reserve, Kent 51°22'59.288"N, 0°46'57.459"E Larvae, pupae F0 Dipping Freshwater pond/drainage ditch
Cx. pipiens (s.l.) West Byfleet, Surrey 51°20'35.084"N, 0°29'56.664"W Eggs F0 Dipping Container
Ae. detritus, Ae. caspius Wallasea Island, Essex 51°36'55.065"N, 0°49'22.066"E Adults F0 CO2 traps Saltmarsh
Ae. rusticus, Ae. cantans/Ae. annulipes Bartley Heath, Hampshire 51°16'32.568"N, 0°57'18.207"W Adults F0 Human landing Woodland/temporary groundwater
Ae. detritus Dee Marsh, Cheshire 53°16'39.48"N, 3°4'5.286"W Larvae, pupae F0 Dipping Saltmarsh
  1. aThe Caldbeck colony was established from filial generation ~f50 at APHA in February 2015
  2. bThe Brookwood colony ~f51 in February 2016
  3. cCollected, colonised and characterised by the Pirbright Institute [22]
  4. Dipping refers to the use of pans to collect larvae using a scooping action to target collection of Aedes spp. or a tilting action allowing the passive flow of water into the pan to target Culex larvae as described previously [50]. The females of Ae. cantans and Ae. annulipes cannot be reliably identified based on morphological traits [51]. Our attempts to molecularly confirm the identity of specimens from Bartley Heath by using the DNA barcoding retrieved ambiguous results for the species; therefore, we treat Ae. cantans/Ae. annulipes in our dataset. Locations are based on survey sites from [22, 52,53,54], a map of locations is presented in Additional file 1: Figure S1