Fig. 1

Sequence characteristics of MEDLE family proteins. a Multiple sequence alignment of MEDLE proteins in C. parvum and C. hominis. The Cp and Ch represent for C. parvum and C. hominis, respectively, and consensus sequence shows the amino acid identity with an asterisk and similarity with a period. The predicted signal peptide is shown in bold and underlined, the transmembrane domain is shaded in green, the predicted O-linked glycosylation sites are in red, while the N-linked glycosylation sites are in red and italicized. The predicted B cell epitopes of MEDLE proteins are shaded in yellow, and the motif “MEDLE” at the C-terminus is shown in bold and double underlined. b Motifs or domains of MEDLE proteins based on predictions in CryptoDB. The LCRs are shown in blue, the predicted signal peptide is shown in yellow, and the motif “MEDLE” is shown in red. The histone chaperone domain is shown in light blue, the ATP-dependent RNA helicase domain is shown in green, and the serine/threonine protein kinase domain is shown in orange. c Phylogenetic relationship of C. parvum and C. hominis MEDLE family proteins using MEGA 7.0.26. The tree was constructed by a maximum likelihood analysis based on evolutionary distances calculated using the position model. Bootstrap values were obtained by running 1000 pseudoreplicates