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Fig. 3 | Parasites & Vectors

Fig. 3

From: Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum

Fig. 3

Biological characteristics of native CpMEDLE-1. a Relative expression levels of the cgd5_4580 gene at various C. parvum culture time as determined by qPCR. Data from the Cryptosporidium 18S rRNA gene were used as an internal control for data normalization. Data presented are mean ± SD from three replicate cultivation assays, with qPCR analysis of each RNA extraction performed in duplicate. b Expression of CpMEDLE-1 on free sporozoites (top panel) and intracellular developmental stages of C. parvum in HCT-8 cell cultures at 24 h (middle panel) and 48 h (bottom panel). The images were taken under differential interference contrast (DIC), with nucleus counter-stained with 4', 6-diamidino-2-phenylindole (DAPI), parasites stained by immunofluorescence with Alexa 594-labled CpMEDLE-1 (MEDLE-1), and superimposition of the three (Merged). Scale-bars: 5 μm. c Neutralization efficiency of post-immune serum against CpMEDLE-1 in C. parvum culture. Hypochlorite-treated oocysts were pre-incubated in medium with 1:1,000, 1:500 and 1:200 dilutions of pre- and post-immune serum, with medium alone as a control. Levels of infection in the presence of post-immune serum were normalized using data from cultures treated with the pre-immune serum. Data presented are mean ± SD from three replicate assays

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