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Table 1 Primer and probe selections following RT-PCR melt curve screening

From: Development of a rapid field-applicable molecular diagnostic for knockdown resistance (kdr) markers in An. gambiae

Primer/probe

Sequence (5'–3')

1014 Forward primer

TCCCCGACCATGATCTGCCAA

1014 Reverse primer

GCACCTGCAAAACAATGTCATGTAA

1014 Probe

MGGAAATTTTGTCGFAAGTAAFGCAAP

1575 Forward primer

AAAGAAAGCTGGTGGATCGC

1575 Reverse primer

TGAAAACACTAACCCTTGGACGA

1575 Probe

MTATTATGCAAFGAAAAAAAFGGGTP

  1. F, M and P (in bold) denote a fluorescein labelled deoxythymidine base, a trimethoxystilbene and propanol conjugate, respectively.
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