Table 2 Comparison of melting temperature of lysate and extracted DNA template
Tm range (°C) | Average Tm ± SD | |||
|---|---|---|---|---|
Allele | DNA extract (n) | Crude lysate (n) | DNA extract | Crude lysate |
1014F | 57.7–58.9 (15) | 59–59.55 (12) | 58.49 ± 0.39 | 59.31 ± 0.14 |
1014L | 52.8–53.7 (13) | 53.9–54.7 (11) | 53.4 ± 0.29 | 54.29 ± 0.21 |