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Table 2 Comparison of melting temperature of lysate and extracted DNA template

From: Development of a rapid field-applicable molecular diagnostic for knockdown resistance (kdr) markers in An. gambiae

  Tm range (°C) Average Tm ± SD
Allele DNA extract (n) Crude lysate (n) DNA extract Crude lysate
1014F 57.7–58.9 (15) 59–59.55 (12) 58.49 ± 0.39 59.31 ± 0.14
1014L 52.8–53.7 (13) 53.9–54.7 (11) 53.4 ± 0.29 54.29 ± 0.21