Day | Injection | Group 1 | Group 2 | Group 3 |
---|---|---|---|---|
0 | First | 100 μg rPAS-5/mouse + adjuvant | 100 μg rGal-1/mouse + adjuvant | PBS + adjuvant |
7 | Second | 50 μg rPAS-5/mouse + adjuvant | 50 μg rGal-1/mouse + adjuvant | PBS + adjuvant |
14 | Third | 50 μg rPAS-5/mouse + adjuvant | 50 μg rGal-1/mouse + adjuvant | PBS + adjuvant |
21 | Sacrifice and collect the samples (0 weeks before infection) | |||
Challenge with 30 A. cantonensis larvae/mouse | ||||
28 | Sacrifice and collect the samples (1 week after infection) | |||
35 | Sacrifice and collect the samples (2 weeks after infection) | |||
42 | Sacrifice and collect the samples (3 weeks after infection) |