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Table 1 Experimental protocol: procedure for immunization of mice against A. cantonensis

From: The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis

Day Injection Group 1 Group 2 Group 3
0 First 100 μg rPAS-5/mouse + adjuvant 100 μg rGal-1/mouse + adjuvant PBS + adjuvant
7 Second 50 μg rPAS-5/mouse + adjuvant 50 μg rGal-1/mouse + adjuvant PBS + adjuvant
14 Third 50 μg rPAS-5/mouse + adjuvant 50 μg rGal-1/mouse + adjuvant PBS + adjuvant
21 Sacrifice and collect the samples (0 weeks before infection)
Challenge with 30 A. cantonensis larvae/mouse
28 Sacrifice and collect the samples (1 week after infection)
35 Sacrifice and collect the samples (2 weeks after infection)
42 Sacrifice and collect the samples (3 weeks after infection)