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Table 1 Experimental protocol: procedure for immunization of mice against A. cantonensis

From: The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis

Day

Injection

Group 1

Group 2

Group 3

0

First

100 μg rPAS-5/mouse + adjuvant

100 μg rGal-1/mouse + adjuvant

PBS + adjuvant

7

Second

50 μg rPAS-5/mouse + adjuvant

50 μg rGal-1/mouse + adjuvant

PBS + adjuvant

14

Third

50 μg rPAS-5/mouse + adjuvant

50 μg rGal-1/mouse + adjuvant

PBS + adjuvant

21

Sacrifice and collect the samples (0 weeks before infection)

Challenge with 30 A. cantonensis larvae/mouse

28

Sacrifice and collect the samples (1 week after infection)

35

Sacrifice and collect the samples (2 weeks after infection)

42

Sacrifice and collect the samples (3 weeks after infection)