msp1
|
Primary
|
M1-OF:
|
CTAGAAGCTTTAGAAGATGCAGTATTG
|
Initial denaturation: 95 °C for 5 min; PCR: 25 cycles of 94 °C for 1 min, 61 °C for 45 s, 72 °C for 1.5 min; final elongation:72 °C for 5 min
|
61 °C
| |
M1-OR:
|
CTTAAATAGTATTCTAATTCAAGTGGATCA
|
Secondary
|
M1- KF:
|
AATGAAGAAGAAATTACTACAAAAGGTGC
|
Initial denaturation: 95 °C for 5 min; PCR: 30 cycles of 94 °C for 45 s, allele-specific °C for 30s, 72 °C for 1 min; final elongation: 72 °C for 5 min
|
K1(61 °C)
|
3D7
|
M1-KR:
|
GCTTGCATCAGCTGGAGGGCTTGCACCAGA
|
M1-MF:
|
AAATGAAGGAACAAGTGGAACAGCTGTTAC
|
MAD20 (61 °C)
|
HB3
|
M1-MR:
|
ATCTGAAGGATTTGTACGTCTTGAATTACC
|
M1-RF:
|
TAAAGGATGGAGCAAATACTCAAGTTGTTG
|
R033 (63 °C)
|
R033
|
M1-RR:
|
CATCTGAAGGATTTGCAGCACCTGGAGATC
|
msp2
|
Primary
|
MSP2-1:
|
ATGAAGGTAATTAAAACATTGTCTATTATA
|
Initial denaturation: 94 °C for 5 min; PCR: 40 cycles of 94 °C for 1.5 min, 55 °C for 45 s, 72 °C for 1.5 min; final elongation: 72 °C for 10 min
|
(55 °C)
| |
MSP2-4:
|
ATATGGCAAAAGATAAAACAAGTGTTGCTG
|
Secondary
|
A1:
|
GCAGAAAGTAAGCCTTCTACTGGTGCT
|
Initial denaturation: 94 °C for 2 min; PCR: 30 cycles of 94 °C for 1.5 min, 55 °C for 45 s, 72 °C for 1.5 min; final elongation: 72 °C for 10 min
|
IC/3D7 (55 °C)
|
3D7
|
3D7 A2:
|
GATTTGTTTCGGCATTATTATGA
|
FC27 B1:
|
GCAAATGAAGGTTCTAATACTAATAG
|
FC27 (55 °C)
|
HB3
|
FC27 B2:
|
GCTTTGGGTCCTTCTTCAGTTGATTC
|