Fig. 5

Expression and transcription of pVAX1-SAG in vivo were identified through RT-PCR and western blot assays. a RT-PCR of pVAX1-EmSAG transcription in chicken muscle. Lane M: DNA marker DL2000; Lanes 1 and 2: the muscle RNA sample from the non-inoculated chicken; Lane 3: the muscle RNA sample from the pVAX1-inoculated chicken; Lane 4: the muscle RNA sample from the pVAX1-EmSAG injected chicken. b Western blot of pVAX1-EmSAG in chicken muscle. Lane M: pre-stained protein marker; Lane 1: the protein sample from pVAX1-inoculated chickens; Lane 2: the protein sample from pVAX1- EmSAG inoculated chickens