Fig. 4
From: A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
Indirect immunofluorescence assays of C6/36 cells infected or not (mock) with 1 MOI BR/P07. At 3 days post-infection, the cell monolayer was fixed and permeabilized with methanol:acetone (1:1) at -20 °C and then incubated with anti-alphavirus monoclonal antibody (mAb) clone 1A4B-6 followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 conjugate. Pictures were taken using a Leica DMI 6000B inverted microscope attached to a Leica DFC365 FX camera, and the images were visualized and processed using the Leica Application Suite Advanced Fluorescence 3.1.0 software