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Table 2 Primers and probes for Gyrodactylus salaris, rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar) used in the present study. The probes used are TaqMan MGB probes with either Fam or Hex reporter dyes

From: Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss)

Target species/gene

Name

Primer/probe

Sequence (5'-3')

Reference

G. salaris/ITS

G.sal208F

Forward

GGTGGTGGCGCACCTATTC

Present study

G.sal149R

Reverse

ACGATCGTCACTCGGAATCGAT

Present study

G.sal188P

Probe

(FAM)CAAGCAGAACTGGTTAAT(MGBNFQ)

Present study

G. salaris/ITS

F

Forward

CGATCGTCACTCGGAATCG

Collins et al. [32]

R

Reverse

GGTGGCGCACCTATTCTACA

Collins et al.[32]

Gsal2

Probe

(FAM)TCTTATTAACCAGTTCTGC(MGBNFQ)

Collins et al. [32]

O. mykiss/cytb

RBTF

Forward

AGTCTCTCCCTGTATATCGTC

Wilcox et al. [35]

RBTR

Reverse

GATTTAGTTCATGAAGTTGCGTGAGTA

Wilcox et al. [35]

RBTP

Probe

(FAM)CCAACAACTCTTTAACCATC(MGBNFQ)

Wilcox et al. [35]

S. salar/cytb

Salmonid Cyt B FOR

Forward

CGGAGCATCTTTCTTCTTTATCTGT

Matejusova et al. [34]

S. salar REV

Reverse

ACTCCGATATTTCAGGTTTCTTTATATAGA

Matejusova et al. [34]

S. salar Cyt B Probe

Probe

(HEX)CCAACAACTCTTTAACCATC-(MGBNFQ)

Matejusova et al. [34]