Table 1 Oligonucleotide sequences of primers and probes used for real-time PCRs and conventional PCRs in this study
From: Detection of bacterial pathogens in clade E head lice collected from Niger’s refugees in Algeria
Target | Name | Primers (5′-3′) and probes | Source |
|---|---|---|---|
Pediculus humanus cytochrome b | Cytb | F_GAGCGACTGTAATTACTAATC | [53] |
R_CAACAAAATTATCCGGGTCC | |||
Acinetobacter spp. RNA polymerase β subunit gene | rpoB | F_TACTCATATACCGAAAAGAAACGG | [51] |
R_GGYTTACCAAGRCTATACTCAAC | |||
FAM-CGCGAAGATATCGGTCTSCAAGC-TAMRA | |||
rpoB (zone1) | F_TAYCGYAAAGAYTTGAAAGAAG | [54] | |
R_CMACACCYTTGTTMCCRTGA | |||
Rickettsia spp. citrate synthase (gltA) | RKNDO3 | F_AATGCTCTTGCAGCTGGTTCT | [55] |
R_TCGAGTGCTAATATTTTTGAAGCA | |||
FAM-CGGTGGTGTTAATGCTGCGTTACAACA-TAMRA | |||
Yersinia pestis plasminogen activator gene | PLA | F_ATGGAGCTTATACCGGAAAC | [56] |
R_GCGATACTGGCCTGCAAG | |||
FAM-TCCCGAAAGGAGTGCGGGTAATAGG-TAMRA | |||
Borrelia spp. 16S ribosomal RNA | Bor16S | F_AGCCTTTAAAGCTTCGCTTGTAG | [57] |
R_GCCTCCCGTAGGAGTCTGG | |||
FAM-CCGGCCTGAGAGGGTGAACGG-TAMRA | |||
Bartonella quintana Hypothetical intracellular effector 3-oxoacyl-synthase gene | yopP | F_TAAACCTCGGGGGAAGCAGA | [58] |
R_TTTCGTCCTCAACCCCATCA | |||
FAM-CGTTGCCGACAAGACGTCCTTG-TAMRA | |||
fabF3 | F_GCGGCCTTGCTCTTGATGA | ||
R_GCTACTCTGCGTGCCTTGGA | |||
FAM-TGCAGCAGGTGGAGAGAACGTG-TAMRA | |||
Anaplasma spp. 23S ribosomal RNA | TtAna | F_TGACAGCGTACCTTTTGCAT | [59] |
R_TGGAGGACCGAACCTGTTAC | |||
FAM-GGATTAGACCCGAAACCAAG-TAMRA | |||
Coxiella burnetii Spacers | IS1111 | F_CAAGAAACGTATCGCTGTGGC | [42] |
R_CACAGAGCCACCGTATGAATC | |||
FAM-CCGAGTTCGAAACAATGAGGGCTG-TAMRA | |||
IS30A | F_ CGCTGACCTACAGAAATATGTCC | ||
R_ GGGGTAAGTAAATAATACCTTCTGG | |||
FAM-CATGAAGCGATTTATCAATACGTGTATGC-TAMRA | |||
Cox2 | F_CAACCCTGAATACCCAAGGA | [34] | |
R_GAAGCTTCTGATAGGCGGGA | |||
Cox5 | F_CAGGAGCAAGCTTGAATGCG | ||
R_TGGTATGACAACCCGTCATG | |||
Cox18 | F_CGCAGACGAATTAGCCAATC | ||
R_TTCGATGATCCGATGGCCTT | |||
Cox22 | F_GGGAATAAGAGAGTTAGCTCA | ||
R_CGCAAATTTCGGCACAGACC |